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专利名称：Automated optical alignment system and
method using Raman scattering of capillarytube contents
发明人：Wilbur I. Kaye,Stephen L. Pentoney, Jr.申请号：US08/409557申请日：19950323公开号：US05614726A公开日：19970325
摘要：A system and method are disclosed for optically aligning a capillary tube and anexcitation laser beam for fluorescence detection applications by utilizing the Ramanscatter signals of the capillary tube's contents. For example, Raman scatter by anelectrophoretic separation matrix may be used for alignment in a capillary
electrophoresis system. Fluorescent material may be present and may also be used foralignment purposes, but is not necessary. The invention employs a parabolic reflector,having apertures through which the capillary tube and the laser beam are guided so thatthey intersect, preferably at right angles and at the focal point of the reflector. TheRaman scatter signals of the material within the capillary tube are collected via a series offilters and this information is used to reposition, if necessary, a focusing lens that directsthe excitation beam into the reflector and the capillary tube, so that the Raman scattersignals are maximized. Maximal Raman scatter signals indicate proper alignment of thecapillary tube and the excitation beam. Other signals, such as fluorescence emission fromthe sample, may then be gathered. Adjustment of the focusing lens may be automatedso that alignment of the capillary tube and the beam is maintained throughout analysis of
the tube's contents. Sequential alignment of an array of capillary tubes with an excitationbeam is also disclosed.
申请人：BECKMAN INSTRUMENTS, INC.
代理人：William H. May,Janis C. Henry
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